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The sample is loaded onto the column, as well as nonpolar elements interact far more strongly With all the nonpolar stationary section, leading to their separation within the polar components.Determined by their affinity to the stationary and mobile phases, analytes partition between The 2 during the separation system taking place in the column. Th
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Tubing with a nano-liquid chromatography (nano-LC) system, utilized for quite lower movement capacities The internal diameter (ID) of the HPLC column is an important parameter.[44] It may possibly affect the detection response when decreased a result of the lowered lateral diffusion with the solute band. It may have an effect on the separation sele
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